Product Name :
anti-smyd2 mouse mab
Isotype :
IgG1/kappa
Conjugate :
Unconjugated
Synonyms:
HSKM-B, KMT3C
UniProt ID :
Q9NRG4
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 ‘Lys-4’ (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates ‘Lys-370’ of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at ‘Lys-860’. Cellular location Cytoplasm, Nucleus
Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 50 kDa ICC/IF Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Anti-SMYD2 Mouse mAb Clone Number: 13147 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: HSKM-B, KMT3C Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HSKM-B, KMT3C UniProt ID Q9NRG4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 ‘Lys-4’ (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates ‘Lys-370’ of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at ‘Lys-860’. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 50 kDa ICC/IF Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
Recombinant Anti-SMYD2 Mouse mAb Clone Number: 13147 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: HSKM-B, KMT3C Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HSKM-B, KMT3C UniProt ID Q9NRG4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 ‘Lys-4’ (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates ‘Lys-370’ of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at ‘Lys-860’. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 50 kDa Observed band size: 50 kDa ICC/IF Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
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