anti-sirt1 mouse mab

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Recombinant Anti-SIRT1 Mouse mAb Clone Number: 10865 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse Synonyms: NAD-dependent protein deacetylase sirtuin-1, SIR2L1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms NAD-dependent protein deacetylase sirtuin-1, SIR2L1 UniProt ID Q96EB6 Immunogen Recombinant human SIRT1 fragment MW (kDa) 110 Specificity Anti-SIRT1 Mouse mAb recognizes endogenous level of SIRT1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background NAD-dependent protein deacetylase sirtuin-1 links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Cellular location Nucleus, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HeLa, N2a Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 110 kDa Observed band size: 110 kDa IHC-P Tissue: Mouse testis Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-SIRT1 Mouse mAb Clone Number: 10865 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse Synonyms: NAD-dependent protein deacetylase sirtuin-1, SIR2L1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms NAD-dependent protein deacetylase sirtuin-1, SIR2L1 UniProt ID Q96EB6 Immunogen Recombinant human SIRT1 fragment MW (kDa) 110 Specificity Anti-SIRT1 Mouse mAb recognizes endogenous level of SIRT1 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background NAD-dependent protein deacetylase sirtuin-1 links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Cellular location Nucleus, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HeLa, N2a Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 110 kDa Observed band size: 110 kDa IHC-P Tissue: Mouse testis Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with