anti-sftpa1 + sftpa2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-SFTPA1 + SFTPA2 Rabbit mAb Clone Number: JRMR-10987-14L Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Collectin-4, COLEC4, Collectin-5, COLEC5, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA2B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Collectin-4, COLEC4, Collectin-5, COLEC5, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA2B UniProt ID Q8IWL2, Q8IWL1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Human SP-A is expressed in alveolar epithelial type II cells and in tracheal and bronchial submucosal gland cells in lung as well as in other tissues. SFTPA1(SP-A1) and SFTPA2(SP-A2) are expressed in the type II cells; SP-A2 is expressed in human tracheal and bronchial submucosal gland cells. The presence of SP-A in non-ciliated bronchiolar epithelial cells has been inconsistent for human lungs, but consistent positive staining of SP-A has been observed in the non-ciliated bronchiolar epithelial cells in rodents. Cellular location Extracellular matrix, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Human lung,2: Rat lung Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 27 kDa Observed band size: 27 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: Mouse lung Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 27 kDa Observed band size: 27 kDa IHC-P Tissue: Human lung Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-SFTPA1 + SFTPA2 Rabbit mAb Clone Number: JRMR-10987-14L Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Collectin-4, COLEC4, Collectin-5, COLEC5, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA2B Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Collectin-4, COLEC4, Collectin-5, COLEC5, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA2B UniProt ID Q8IWL2, Q8IWL1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Human SP-A is expressed in alveolar epithelial type II cells and in tracheal and bronchial submucosal gland cells in lung as well as in other tissues. SFTPA1(SP-A1) and SFTPA2(SP-A2) are expressed in the type II cells; SP-A2 is expressed in human tracheal and bronchial submucosal gland cells. The presence of SP-A in non-ciliated bronchiolar epithelial cells has been inconsistent for human lungs, but consistent positive staining of SP-A has been observed in the non-ciliated bronchiolar epithelial cells in rodents. Cellular location Extracellular matrix, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Human lung,2: Rat lung Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 27 kDa Observed band size: 27 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: Mouse lung Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 27 kDa Observed band size: 27 kDa IHC-P Tissue: Human lung Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with