anti-septin 7 mouse mab

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Recombinant Anti-Septin 7 Mouse mAb Clone Number: 10145 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: SEPTIN7, CDC10, SEPT7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms SEPTIN7, CDC10, SEPT7 UniProt ID Q16181 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background SEPT7 is a protein that in humans is encoded by the SEPT7 gene. It is required for normal organization of the actin cytoskeleton and normal progress through mitosis. SEPT7 forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 51 kDa Observed band size: 51 kDa ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-Septin 7 Mouse mAb Clone Number: 10145 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: SEPTIN7, CDC10, SEPT7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms SEPTIN7, CDC10, SEPT7 UniProt ID Q16181 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background SEPT7 is a protein that in humans is encoded by the SEPT7 gene. It is required for normal organization of the actin cytoskeleton and normal progress through mitosis. SEPT7 forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: NIH/3T3 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 51 kDa Observed band size: 51 kDa ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with