anti-alpha-synuclein rabbit mab

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Recombinant Rabbit Monoclonal Anti-alpha-Synuclein Rabbit mAb Clone Number: JRMR-10752-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: SNCA, NACP, PARK1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SNCA, NACP, PARK1 UniProt ID P37840 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Alpha-synuclein may serve to integrate presynaptic signaling and membrane trafficking. Defects in this protein have been implicated in the pathogenesis of Parkinson disease. Alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer’s disease. Cellular location Cytoplasm, Membrane, Nucleus, Cell junction, Synapse, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse brain, 2: Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 18 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: U-87 MGProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 18 kDa ICC/IF Cell line: U-87 MGFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-alpha-Synuclein Rabbit mAb Clone Number: JRMR-10752-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: SNCA, NACP, PARK1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SNCA, NACP, PARK1 UniProt ID P37840 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Alpha-synuclein may serve to integrate presynaptic signaling and membrane trafficking. Defects in this protein have been implicated in the pathogenesis of Parkinson disease. Alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer’s disease. Cellular location Cytoplasm, Membrane, Nucleus, Cell junction, Synapse, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse brain, 2: Rat brainProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 18 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: U-87 MGProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 18 kDa ICC/IF Cell line: U-87 MGFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with