anti-ras mouse mab

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Recombinant Anti-Ras Mouse mAb Clone Number: JMMR-3300 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: GTPase Hras, H-Ras-1, HRAS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms GTPase Hras, H-Ras-1, HRAS UniProt ID P01112 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ras is a 21 kDa guanine-nucleotide binding proteins. Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. Cellular location Cell membrane, Golgi apparatus, Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Mouse brain, 2: Rat brain, 3: Neuro-2a, 4: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 21 kDa Observed band size: 21 kDa IHC-P Tissue: Human kidney Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-Ras Mouse mAb Clone Number: JMMR-3300 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: GTPase Hras, H-Ras-1, HRAS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms GTPase Hras, H-Ras-1, HRAS UniProt ID P01112 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ras is a 21 kDa guanine-nucleotide binding proteins. Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. Cellular location Cell membrane, Golgi apparatus, Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Mouse brain, 2: Rat brain, 3: Neuro-2a, 4: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 21 kDa Observed band size: 21 kDa IHC-P Tissue: Human kidney Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with