anti-propionyl-histone h4 (lys16) mouse mab

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Anti-Propionyl-Histone H4 (Lys16) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K16pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys16) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys16) Mouse mAb detects histone H4 only when it is propionylated at Lys16. This antibody has been shown to selectively recognize acetylated H4 peptide at Lys16, but not the structurally similar acetylated, butyrylated, crotonylated peptide at Lys16 or unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:200 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation,phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys16 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+Sodium propionate (10mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-Propionyl-Histone H4 (Lys16) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K16pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys16) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys16) Mouse mAb detects histone H4 only when it is propionylated at Lys16. This antibody has been shown to selectively recognize acetylated H4 peptide at Lys16, but not the structurally similar acetylated, butyrylated, crotonylated peptide at Lys16 or unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:200 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation,phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys16 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+Sodium propionate (10mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with