anti-propionyl-histone h4 (lys12) mouse mab

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Anti-Propionyl-Histone H4 (Lys12) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H4K12pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys12) Mouse mAb detects histone H4 only when it is propionylated at Lys12. This antibody has been shown to selectively recognize acetylated H4 peptide at Lys12, but not the structurally similar acetylated, butyrylated, crotonylated peptide at Lys12 or unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kpr), structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys12 may play a vital role in the epigenetic modulation, includingchromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Peptide amount: 1 ng, 4 ng, 16 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa; (+) HeLa + sodium propionate (10 mM, 4 hours) Protein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human spleen Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Anti-Propionyl-Histone H4 (Lys12) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H4K12pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12pr UniProt ID P62805 Immunogen Propionylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-Propionyl-Histone H4 (Lys12) Mouse mAb detects histone H4 only when it is propionylated at Lys12. This antibody has been shown to selectively recognize acetylated H4 peptide at Lys12, but not the structurally similar acetylated, butyrylated, crotonylated peptide at Lys12 or unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kpr), structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys12 may play a vital role in the epigenetic modulation, includingchromatin remodeling and transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Peptide amount: 1 ng, 4 ng, 16 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa; (+) HeLa + sodium propionate (10 mM, 4 hours) Protein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human spleen Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with