anti-propionyl-histone h3 (lys23) rabbit pab

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Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23pr UniProt ID P68431 Immunogen Propionylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb detects histone H3 only when it is propionylated at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Rat ChIP 6μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, and others. Among these modifications, lysine propionylation (Kprop) has emerged as a newly identified reversible modification that plays a crucial role in controlling protein activity. Structurally similar to lysine acetylation, lysine propionylation is prevalent in both prokaryotes and eukaryotes and has been identified in a wide range of proteins, including histones and non-histone substrates like p53. Recent research has unveiled the involvement of stoichiometric complexes comprising lysine acetyltransferase 6A (KAT6A), KAT6B, and bromodomain- and PHD finger-containing protein 1 (BRPF1) in the catalysis of histone H3 Lys23 propionylation (H3K23pr). Importantly, deficiencies in histone propionylation have been associated with developmental disorders and cancer. Cellular location Nucleus Images Dot Blot Peptide amount: 1ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23pr, Lane 2: H3K18ac, Lane 3: H3K23ac, Lane 4: H3K23buLane 5: H3K23cr, Lane 6: H3K27ac, Lane 7: H3K27bu, Lane 8: H3K23un WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium propionate (10 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 17 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23pr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23pr UniProt ID P68431 Immunogen Propionylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb detects histone H3 only when it is propionylated at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Rat ChIP 6μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, and others. Among these modifications, lysine propionylation (Kprop) has emerged as a newly identified reversible modification that plays a crucial role in controlling protein activity. Structurally similar to lysine acetylation, lysine propionylation is prevalent in both prokaryotes and eukaryotes and has been identified in a wide range of proteins, including histones and non-histone substrates like p53. Recent research has unveiled the involvement of stoichiometric complexes comprising lysine acetyltransferase 6A (KAT6A), KAT6B, and bromodomain- and PHD finger-containing protein 1 (BRPF1) in the catalysis of histone H3 Lys23 propionylation (H3K23pr). Importantly, deficiencies in histone propionylation have been associated with developmental disorders and cancer. Cellular location Nucleus Images Dot Blot Peptide amount: 1ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23pr, Lane 2: H3K18ac, Lane 3: H3K23ac, Lane 4: H3K23buLane 5: H3K23cr, Lane 6: H3K27ac, Lane 7: H3K27bu, Lane 8: H3K23un WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium propionate (10 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 17 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with