anti-phospho-ikb alpha (ser36) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-IkB alpha (Ser36) Rabbit mAb Clone Number: JRMR-10753-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: NF-kappa-B inhibitor alpha, NFKBIA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms NF-kappa-B inhibitor alpha, NFKBIA UniProt ID P25963 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000-2000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa + Calyculin A (100ng/ml, 30min)+TNF-α (20ng/ml, 5min)Protein loading amount: 20 μgExposure time: 10 sPredicted band size: 36 kDaObserved band size: 39 kDa :
Recombinant Rabbit Monoclonal Anti-Phospho-IkB alpha (Ser36) Rabbit mAb Clone Number: JRMR-10753-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: NF-kappa-B inhibitor alpha, NFKBIA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms NF-kappa-B inhibitor alpha, NFKBIA UniProt ID P25963 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000-2000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa + Calyculin A (100ng/ml, 30min)+TNF-α (20ng/ml, 5min)Protein loading amount: 20 μgExposure time: 10 sPredicted band size: 36 kDaObserved band size: 39 kDa