anti-phospho-hsf1 (ser326) rabbit pab

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Anti-Phospho-HSF1 (Ser326) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: HSTF 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSTF 1 UniProt ID Q00613 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HSF1 belongs to heat-shock transcription factor that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishs a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa that likely represent different phosphorylation states. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa treated with heat at 43 °C for 30 minutesProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 57 kDaObserved band size: 82 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Cell lysates:HeLa cell treated with heat at 43 °C for 30 minutes , then the membrane treated with Lambda protein phosphatase (400 U/ml) at 30 °C for 1 hour; 2: Cell lysates:HeLa cell treated with heat at 43 °C for 30 minutes, without lambda protein phosphatase treatmentProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 57 kDaObserved band size: 82 kDa :
Anti-Phospho-HSF1 (Ser326) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: HSTF 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSTF 1 UniProt ID Q00613 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HSF1 belongs to heat-shock transcription factor that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishs a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa that likely represent different phosphorylation states. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa treated with heat at 43 °C for 30 minutesProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 57 kDaObserved band size: 82 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Cell lysates:HeLa cell treated with heat at 43 °C for 30 minutes , then the membrane treated with Lambda protein phosphatase (400 U/ml) at 30 °C for 1 hour; 2: Cell lysates:HeLa cell treated with heat at 43 °C for 30 minutes, without lambda protein phosphatase treatmentProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 57 kDaObserved band size: 82 kDa