anti-phospho-histone h2a.x (ser139) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-Histone H2A.X (Ser139) Rabbit mAb Clone Number: JRMR-10758-23C Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:5000 Human, Mouse IHC-P 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. H2A histone family member X (usually abbreviated as H2A.x) is a type of histone protein from the H2A family encoded by the H2AFX gene. H2A.x contributes to nucleosome-formation, chromatinremodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa + Nocodazole (100ng-ml, 18h) + CalyculinA(100nM,1h)Protein loading amount: 20 μgExposure time: 3 s Predicted band size: 15 kDa Observed band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): NIH-3T3, (+): NIH-3T3 + Nocodazole (100ng-ml,18hr) + CalyculinA(100nM,1h)Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Phospho-Histone H2A.X (Ser139) Rabbit mAb Clone Number: JRMR-10758-23C Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:5000 Human, Mouse IHC-P 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. H2A histone family member X (usually abbreviated as H2A.x) is a type of histone protein from the H2A family encoded by the H2AFX gene. H2A.x contributes to nucleosome-formation, chromatinremodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa + Nocodazole (100ng-ml, 18h) + CalyculinA(100nM,1h)Protein loading amount: 20 μgExposure time: 3 s Predicted band size: 15 kDa Observed band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): NIH-3T3, (+): NIH-3T3 + Nocodazole (100ng-ml,18hr) + CalyculinA(100nM,1h)Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with