anti-phospho-histone h2a.x (ser139) mouse mab

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Anti-Phospho-Histone H2A.X (Ser139) Mouse mAb Clone Number: 12354 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: HeLa untreated (Left) or treated with Okadaic acid (100nM, 1hr) + Calyculin A(100nM, 1hr) (Right)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRLProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 15 kDaObserved band size: 15 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-Phospho-Histone H2A.X (Ser139) Mouse mAb Clone Number: 12354 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: HeLa untreated (Left) or treated with Okadaic acid (100nM, 1hr) + Calyculin A(100nM, 1hr) (Right)Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRLProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 15 kDaObserved band size: 15 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with