anti-phospho-histone h2a.x (ser139) mouse mab

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Anti-Phospho-Histone H2A.X (Ser139) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IP Reactivity: Human, Mouse, Rat Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IP 1:25 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa IP IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extractsIP Ab incubation condition: :
Anti-Phospho-Histone H2A.X (Ser139) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IP Reactivity: Human, Mouse, Rat Synonyms: H2A.XS139ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2A.XS139ph UniProt ID P16104 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IP 1:25 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa IP IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extractsIP Ab incubation condition: