anti-phospho-egfr (ser1166) rabbit pab

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Anti-Phospho-EGFR (Ser1166) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: ERBB1, HER1, SA7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ERBB1, HER1, SA7 UniProt ID P00533 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) is implicated in many functions, including maintaining the active state enzyme, providing a binding surface for substrate proteins, creates a major docking site for the adaptor protein. EGFR S1166 phosphorylation induced by a combination of EGF and gefitinib has a potentially negative impact on lung cancer cell growth. Cellular location Nucleus, Endoplasmic reticulum membrane, Cell membrane, Golgi apparatus membrane, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): A431, (+): A431+pervanadate(1mM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 180kDa Observed band size: 180kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): A431, (+): A431+EGF(100ng/ml, 5min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 180kDa Observed band size: 180kDa :
Anti-Phospho-EGFR (Ser1166) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: ERBB1, HER1, SA7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms ERBB1, HER1, SA7 UniProt ID P00533 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) is implicated in many functions, including maintaining the active state enzyme, providing a binding surface for substrate proteins, creates a major docking site for the adaptor protein. EGFR S1166 phosphorylation induced by a combination of EGF and gefitinib has a potentially negative impact on lung cancer cell growth. Cellular location Nucleus, Endoplasmic reticulum membrane, Cell membrane, Golgi apparatus membrane, Endosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): A431, (+): A431+pervanadate(1mM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 180kDa Observed band size: 180kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): A431, (+): A431+EGF(100ng/ml, 5min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 180kDa Observed band size: 180kDa