anti-pd1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-PD1 Rabbit mAb Clone Number: JRMR-10011-6SH2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： IHC-P ICC/IF Reactivity: Human Synonyms: Programmed cell death protein 1, PDCD1, Protein PD-1, hPD-1, CD279 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Programmed cell death protein 1, PDCD1, Protein PD-1, hPD-1, CD279 UniProt ID Q15116 Immunogen Synthetic peptide derived from the human PD1 protein MW (kDa) Synthetic peptide derived from the human PD1 protein Specificity Anti-PD1 Rabbit mAb detects endogenous levels of total PD-1 protein. Product Usage Information Applications Dilution Recommended Species IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The programmed cell death 1 protein (PD1) is an inhibitory receptor expressed on antigen activated T-cells that plays a critical role in the induction and maintenance of immune tolerance to self-antigens. Upon binding to its ligands CD274/PDCD1L1 (PD-L1) and CD273/PDCD1LG2 (PD-L2), PD1 is phosphorylated within its immunoreceptor tyrosine-based switch motif (ITSM), which facilitates the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2. PTPN11/SHP-2 then dephosphorylates key T-cell receptor (TCR) proximal signaling molecules, including ZAP70, PRKCQ/PKC theta and CD247/CD3 zeta, leading to the direct inhibition of T-cell activation. The PD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and evade destruction by the immune system, thereby facilitating tumor survival. Cellular location Membrane Images IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-PD1 Rabbit mAb Clone Number: JRMR-10011-6SH2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： IHC-P ICC/IF Reactivity: Human Synonyms: Programmed cell death protein 1, PDCD1, Protein PD-1, hPD-1, CD279 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Programmed cell death protein 1, PDCD1, Protein PD-1, hPD-1, CD279 UniProt ID Q15116 Immunogen Synthetic peptide derived from the human PD1 protein MW (kDa) Synthetic peptide derived from the human PD1 protein Specificity Anti-PD1 Rabbit mAb detects endogenous levels of total PD-1 protein. Product Usage Information Applications Dilution Recommended Species IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The programmed cell death 1 protein (PD1) is an inhibitory receptor expressed on antigen activated T-cells that plays a critical role in the induction and maintenance of immune tolerance to self-antigens. Upon binding to its ligands CD274/PDCD1L1 (PD-L1) and CD273/PDCD1LG2 (PD-L2), PD1 is phosphorylated within its immunoreceptor tyrosine-based switch motif (ITSM), which facilitates the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2. PTPN11/SHP-2 then dephosphorylates key T-cell receptor (TCR) proximal signaling molecules, including ZAP70, PRKCQ/PKC theta and CD247/CD3 zeta, leading to the direct inhibition of T-cell activation. The PD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and evade destruction by the immune system, thereby facilitating tumor survival. Cellular location Membrane Images IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with