anti-o-linked n-acetylglucosamine rabbit mab

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Recombinant Rabbit Monoclonal Anti-O-Linked N-Acetylglucosamine Rabbit mAb Clone Number: 17H2L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: O-GlcNAc Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms O-GlcNAc UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background O-Linked β-N-acetylglucosamine modification (GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-linked glycosidic bond and is found mostly within the cytoplasm or nucleoplasm. GlcNAcylation regulates several important cellular processes, such as signal transduction, protein expression, degradation, embryonic stem cell pluripotency and trafficking. This post-translational modification is regulated by OGT and O-GlcNAcase (β-N-acetylglucosaminidase) enzymes, whereas OGT catalyzes the attachment and O-GlcNAcase catalyzes the removal of O-GlcNAc to proteins. O-GlcNAc modification has been observed on histones: H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+PUGNAC (100μM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: BRL, Mouse liver, Mouse colonProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-O-Linked N-Acetylglucosamine Rabbit mAb Clone Number: 17H2L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: O-GlcNAc Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms O-GlcNAc UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background O-Linked β-N-acetylglucosamine modification (GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-linked glycosidic bond and is found mostly within the cytoplasm or nucleoplasm. GlcNAcylation regulates several important cellular processes, such as signal transduction, protein expression, degradation, embryonic stem cell pluripotency and trafficking. This post-translational modification is regulated by OGT and O-GlcNAcase (β-N-acetylglucosaminidase) enzymes, whereas OGT catalyzes the attachment and O-GlcNAcase catalyzes the removal of O-GlcNAc to proteins. O-GlcNAc modification has been observed on histones: H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+PUGNAC (100μM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: BRL, Mouse liver, Mouse colonProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: Multiple kDaObserved band size: Multiple kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with