anti-mono-methyl-histone h3 (lys27) mouse mab

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Recombinant Rabbit Monoclonal Anti-Mono-Methyl-Histone H3 (Lys27) Mouse mAb Clone Number: 12466 Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K27me1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms H3K27me1 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidneyProtein loading quantity: 20 μgExposure time: 30 sPredicted MW: 15 kDaObserved MW: 15 kDa ChIP Cell type: HeLaCross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μl ofProtein A/G MagBeadsComment: The ChIP was performed with 6 μg of normal mouse IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, RAB20 and LDHA-promoter. Data are presented as enrichment of each sample relative to totalamount of input chromatin at each amplicon. :
Recombinant Rabbit Monoclonal Anti-Mono-Methyl-Histone H3 (Lys27) Mouse mAb Clone Number: 12466 Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K27me1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms H3K27me1 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidneyProtein loading quantity: 20 μgExposure time: 30 sPredicted MW: 15 kDaObserved MW: 15 kDa ChIP Cell type: HeLaCross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 μgBeads type and amount per IP: 50 μl ofProtein A/G MagBeadsComment: The ChIP was performed with 6 μg of normal mouse IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, RAB20 and LDHA-promoter. Data are presented as enrichment of each sample relative to totalamount of input chromatin at each amplicon.