anti-mono-methyl-histone h3 (arg8) mouse mab

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Recombinant Rabbit Monoclonal Anti-Mono-Methyl-Histone H3 (Arg8) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P IP ICC/IF Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: H3R8me1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3R8me1 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat，African green monkey IHC-P 1:50-1:200 Human，Mouse，Rat IP 1:25-1:100 Mouse ICC/IF 1:50 Human，Mouse Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: MCF-7, H1299, JK, N2a, COS-7Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: Mouse spleen, Mouse heart, Mouse kidneyProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Mono-Methyl-Histone H3 (Arg8) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P IP ICC/IF Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: H3R8me1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3R8me1 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat，African green monkey IHC-P 1:50-1:200 Human，Mouse，Rat IP 1:25-1:100 Mouse ICC/IF 1:50 Human，Mouse Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: MCF-7, H1299, JK, N2a, COS-7Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: Mouse spleen, Mouse heart, Mouse kidneyProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 17 kDaObserved MW: 17 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for