anti-mcm2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: 13616 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen Recombinant human MCM2 fragment MW (kDa) 130 Specificity Anti-MCM2 Rabbit mAb recognizes endogeneous level of MCM2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Cellular location Chromosome, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate: 1: HeLa, 2: Jurkat, 3: C6, 4: 4T1 Protein loading quantity: 20 μg Exposure time: 1 s Predicted band size: 102 kDa Observed band size: 130 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: 13616 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen Recombinant human MCM2 fragment MW (kDa) 130 Specificity Anti-MCM2 Rabbit mAb recognizes endogeneous level of MCM2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Cellular location Chromosome, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate: 1: HeLa, 2: Jurkat, 3: C6, 4: 4T1 Protein loading quantity: 20 μg Exposure time: 1 s Predicted band size: 102 kDa Observed band size: 130 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with