anti-mapkapk-2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-MAPKAPK-2 Rabbit mAb Clone Number: JRMR-10947-42 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: MAP kinase-activated protein kinase 2, MK-2, MK2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MAP kinase-activated protein kinase 2, MK-2, MK2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2 UniProt ID P49137 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated in response to cytokines, stress, and chemotactic factors. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa, 2: 293T Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 46 kDa Observed band size: 46 kDa :
Recombinant Rabbit Monoclonal Anti-MAPKAPK-2 Rabbit mAb Clone Number: JRMR-10947-42 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: MAP kinase-activated protein kinase 2, MK-2, MK2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MAP kinase-activated protein kinase 2, MK-2, MK2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2 UniProt ID P49137 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated in response to cytokines, stress, and chemotactic factors. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa, 2: 293T Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 46 kDa Observed band size: 46 kDa