Product Name :
anti-map1lc3a rabbit mab
Isotype :
IgG/kappa
Conjugate :
Unconjugated
Synonyms:
MAP1A/MAP1B LC3 A
UniProt ID :
Q9H492
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. Two transcript variants encoding different isoforms have been found for this gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. MAPLC3A is one of the mammalian homologues of yeast ATG8, an important marker and effector of autophagy. Cellular location Cytoplasm, Membrane, Microtubule
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat brain, 2: Mouse brain, 3: Mouse cerebellum, 4: Rat cerebellumProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 16 kDa IHC-P Tissue: Human brainSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-MAP1LC3A Rabbit mAb Clone Number: 11868 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: MAP1A/MAP1B LC3 A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms MAP1A/MAP1B LC3 A UniProt ID Q9H492 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. Two transcript variants encoding different isoforms have been found for this gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. MAPLC3A is one of the mammalian homologues of yeast ATG8, an important marker and effector of autophagy. Cellular location Cytoplasm, Membrane, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat brain, 2: Mouse brain, 3: Mouse cerebellum, 4: Rat cerebellumProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 16 kDa IHC-P Tissue: Human brainSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-MAP1LC3A Rabbit mAb Clone Number: 11868 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: MAP1A/MAP1B LC3 A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms MAP1A/MAP1B LC3 A UniProt ID Q9H492 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. Two transcript variants encoding different isoforms have been found for this gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. MAPLC3A is one of the mammalian homologues of yeast ATG8, an important marker and effector of autophagy. Cellular location Cytoplasm, Membrane, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat brain, 2: Mouse brain, 3: Mouse cerebellum, 4: Rat cerebellumProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 16 kDa IHC-P Tissue: Human brainSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
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