anti-malonyllysine rabbit pab

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Anti-Malonyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kmal Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmal UniProt ID / Immunogen Malonylated lysine peptides MW (kDa) Multiple Specificity Anti-Malonyllysine Rabbit pAb detects proteins with malonylated lysine residues. This pan antibody recognizes malonylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed across various histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms. Cellular location / Images Dot Blot Peptides/Protein amount: 100 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides/proteins used in the experiment is provided below.Dot 1: Malonylated peptide library. Dot 2: Malonylated BSA. Dot 3: Succinylated peptide library. Dot 4: Succinylated BSA. Dot 5: Propionylated peptide library. Dot 6: Propionylated BSA.Dot 7: Butyrylated peptide library. Dot 8: Butyrylated BSA. Dot 9: Unmodified peptide library. Dot 10: BSA. WB Lysates: (-) HeLa; (+) HeLa + sodium malonylate (20 mM, 72 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple ICC/IF Samples: (A) HeLa cells; (B) HeLa cells + sodium malonylate (20 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Anti-Malonyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kmal Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmal UniProt ID / Immunogen Malonylated lysine peptides MW (kDa) Multiple Specificity Anti-Malonyllysine Rabbit pAb detects proteins with malonylated lysine residues. This pan antibody recognizes malonylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed across various histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms. Cellular location / Images Dot Blot Peptides/Protein amount: 100 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides/proteins used in the experiment is provided below.Dot 1: Malonylated peptide library. Dot 2: Malonylated BSA. Dot 3: Succinylated peptide library. Dot 4: Succinylated BSA. Dot 5: Propionylated peptide library. Dot 6: Propionylated BSA.Dot 7: Butyrylated peptide library. Dot 8: Butyrylated BSA. Dot 9: Unmodified peptide library. Dot 10: BSA. WB Lysates: (-) HeLa; (+) HeLa + sodium malonylate (20 mM, 72 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple ICC/IF Samples: (A) HeLa cells; (B) HeLa cells + sodium malonylate (20 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with