anti-malonyllysine mouse mab

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Anti-Malonyllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Kmal Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmal UniProt ID / Immunogen Malonylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed acrossvarious histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 4 ng, 20 ng, 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: JK, 293T Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa :
Anti-Malonyllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Kmal Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmal UniProt ID / Immunogen Malonylated lysine peptides MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed acrossvarious histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Immunogen peptide quantity: 4 ng, 20 ng, 100 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: JK, 293T Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: Multiple kDa Observed band size: Multiple kDa