Transferases. Semi-quantitative true time RT-PCR was performed on RNA isolated from pak choi sprouts treated with methyl jasmonate, jasmonic acid, linolenic acid, and methyl salicylate, and had been compared to respective control therapies. Certain amplification goods were detectable with all selected oligonucleotide primer combinations. Table 1. Oligonucleotide primers made use of for gene expression evaluation by semi-quantitative realtime RT-PCR.Gene function (Gene name) Actin2 (ACT2) Oligonucleotide abbreviation Br-Af Br-Br Br-Myb34f1 Br-Myb34r1 Transcription issue Altered Tryptophan Regulation 1 (ATR1/MYB34) Br-Myb34f2 Br-Myb34r1 Br-Myb34f2 Br-Myb34r2 Br-Myb51f1 Br-Myb51r1 Br-Myb51f2 Br-Myb51r2 Br-Myb51f3 Br-Myb51r2 Br-Myb51f1 Br-Myb51r3 TCCGAAACCAGCAAATCAGAAA Transcription aspect High Indolic Glucosinolate two (HIG2/MYB122) Br-Myb122f1 Br-Myb122r1 Br-Myb122f2 Br-Myb122r2 TGGATGAATCTTCTTCAGACAAT ATGTCGCTTAATGATAGCCACC TGGATGAATCTTGTTTGGAGAAA ACTATGTGTAGTGATAGTCGTG FJ584300 (BrMYB122_1) Bra008131 (BrMYB122_2) Sequence ACGTGGACATCAGGAAGGAC CTTGGTGCAAGTGCTGTGAT GAAGGAATAAAGAAAGGAGCC GCATCTCTTCAATCCAGCTTTT AGAAGGAATAAAGAAAGGAGCT Accession (Gene abbr.) AC189447 (BrACT2) FJ584293 (BrMYB34_1) FJ584294 (BrMYB34_2) FJ584295 (BrMYB34_3) FJ584296 (BrMYB51_1) FJ584297 (BrMYB51_2) FJ584299 (BrMYB51_3) FJ584298 (BrMYB51_4)CATCTCTTCAATCCAGCTTTC ACCGATAACGAAATCAAGAACTA CTCACAAGAACATATCAGAAAAT T ACCGATAACGAAATCAAGAACCA TCCGACAAATCAGAAAACCTCC ACCGATAACGAAATCAAGAACCTTranscription aspect Higher Indolic Glucosinolate 1 (HIG1/MYB51)Int. J. Mol. Sci. 2013, 14 Table 1. Cont.Gene function (Gene name) Cytochrom P450 monooxygenase household 79B (CYP79B) Oligonucleotide abbreviation Br-Cyp79B2f3 Br-Cyp79B2r3 Br-Cyp79B2f4 Br-Cyp79B2r4 Br-Cyp79B3f Br-Cyp79B3r Sulfotransferase 16 (SOT16) Cytochrom P450 monooxygenase family 81F (CYP81F) Br-SOT16f2 Br-SOT16r2 Br-Cyp81F1f Br-Cyp81F1r Br-Cyp81F2f Br-Cyp81F2r Br-Cyp81F3f1 Br-Cyp81F3r1 Br-Cyp81F3f2 Br-Cyp81F3r2 Br-Cyp81F4f1 Br-Cyp81F4r1 Br-Cyp81F4f2 Br-Cyp81F4r2 O-methyltransferase family protein (OMT) Br-OMTf1 Br-OMTr1 Br-OMTf2 Br-OMTr2 Sequence GTTCTCTGAAAACACTGCAGCG TGCTTTTTGTATATCTGATTATCT AC GTTCTCTGAAAACACCGCACCT CTCCTTTTGTATCTCTGATTATCTA TTCTCGGAGAAAACCAAAACC TGCGTTTTGTGTATCGGACTA TTCAAGACGGCAAGAACCAG GGGTCAGCAGCTAGCGAG TCCCTCGCACGCCGACG AGGATGCGGCAGCGAGTTA TCTCCTTCTGAAGATCTCAAAA AGAAAAAGAAGCAGCGAACAC GCCGAGATCACCGATGGAA GCGGAGGAGAAGACGTTCA GCCAAGATCGACGACAGAC TCGGAGAAGGAGGAGAAGAC TTAACGGAAGAGGACATCAAAG AAAGAGGGGAAGGAGACAAAGA TTAACAGTAGAGGACATCAAGA TGGAGGAGAAGGAGAAAAGGA GGCTGTACCGGAGAGACGA GCCGTTCTCATCAAGTGGGTG GGCTGTACCGGAGAGACCC AACGTTTTCATCAAGTGGGTCTAccession (Gene abbr.Tramiprosate ) FJ376045 (BrCYP79B2_1) FJ376047 (BrCYP79B2_2) FJ376047 (BrCYP79B3) FJ376059 (BrSOT16) KBrB006J12.Hesperidin 9 (BrCYP81F1) KBrB027E01.PMID:24513027 six (BrCYP81F2) KBrB006J12.six (BrCYP81F3_1) KBrH064I20.two (BrCYP81F3_2) KBrB006J12.7 (BrCYP81F4_1) KBrH064I20.1 (BrCYP81F4_2) Bra017700 (BrOMT1_1) Bra017699 (BrOMT1_2)It really is already recognized from experiments using a. thaliana that AtMYB34 and AtMYB51 are transcriptionally induced by methyl jasmonate [31]. Our final results in pak choi sprouts are constant with these findings. In detail, methyl jasmonate and jasmonate led to powerful increases of BrMYB34 depending on the isoform, and also the tested isoforms of BrMYB51 are upregulated beneath these circumstances (Figure 5). The treatments with linolenic acid and methyl salicylate also enhanced the expression of precise isoforms of BrMYB34 and BrMYB51 on pak choi sprouts, even so, the increase was less pron.
