anti-lats1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-LATS1 Rabbit mAb Clone Number: JMMR-3221 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: WARTS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms WARTS UniProt ID O95835 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2500 Human, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The Large tumor suppressor 1(LATS1) is a serine / threonine kinases that belong to the NDR family. Human LATS1 was shown to localize to the centrosome and the mitotic spindle and control G2 / M transition by negatively regulating cdc2 kinase activity. LATS1 is also reported to play a role in the G1 tetraploidy checkpoint, via control of p53 expression. LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis. Decreased expression of LATS1 is associated with breast tumor aggressiveness, and mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas. Moreover, LATS1 knockout mice develop soft-tissue sarcomas, ovarian stromal cell tumor, and display a high sensitivity to carcinogenic treatments. Cellular location Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2500 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: K562 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 127 kDa Observed band size: 130 kDa :
Recombinant Rabbit Monoclonal Anti-LATS1 Rabbit mAb Clone Number: JMMR-3221 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: WARTS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms WARTS UniProt ID O95835 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2500 Human, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The Large tumor suppressor 1(LATS1) is a serine / threonine kinases that belong to the NDR family. Human LATS1 was shown to localize to the centrosome and the mitotic spindle and control G2 / M transition by negatively regulating cdc2 kinase activity. LATS1 is also reported to play a role in the G1 tetraploidy checkpoint, via control of p53 expression. LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis. Decreased expression of LATS1 is associated with breast tumor aggressiveness, and mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas. Moreover, LATS1 knockout mice develop soft-tissue sarcomas, ovarian stromal cell tumor, and display a high sensitivity to carcinogenic treatments. Cellular location Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2500 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: K562 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 127 kDa Observed band size: 130 kDa