Hesis inside the model program A. nidulans, we performed a mutagenesis inside a deletion veA strain to create revertant mutants that regained the capacity to make toxin [40]. A number of revertant mutants (RM) have been obtained. In the present study we characterized one particular of those selected revertants, RM7. This revertant mutant presented a point mutation in a gene that we denominated mtfA (master transcription issue A) encoding a novel putative C2H2 zinc finger domain kind transcription element. We show that the mtfA effect on ST production is veAdependent. Furthermore, mtfA regulates the expression of other secondary metabolite gene clusters, for instance those of terrequinone and PN. Moreover, mtfA is also significant for normal sexual and asexual improvement in a. nidulans.Strain name FGSC4 RDAE206 RDAEp206 RAV1 RAV1p RAV2 RM7 RM7p RM7-R2 RM7p-R2 RM7-R2-com RJMP1.49 TRV50.1 TRV50.two TRVDmtfA TRVpDmtfA TRVDmtfA-com TRV60 TDAEDmtfA TDAEpDmtfA RJW41.A RDIT2.3 RJW46.4 RSD10.1 RSD11.two TSD12.Pertinent genotype Wild variety (veA+) yA2, pabaA1, pyrG89; argB2, DstcE::argB, DveA::argB yA2; DstcE::argB, DveA::argB yA2, pabaA1, pyrG89; wA3; argB2, DstcE::argB; veA1 yA2; wA3; DstcE::argB; veA1 yA2; wA3; argB2, DstcE::argB; pyroA4; veA1 yA2, pabaA1, pyrG89; argB2, DstcE::argB, DveA ::argB, mtfA2 yA2, DstcE::argB, DveA ::argB, mtfA2 yA2, pyrG89; wA3; argB2, DstcE::argB, mtfA2, veA1 yA2; wA3; DstcE::argB, mtfA2, veA1 yA2, pyrG89; wA3; argB2, DstcE::argB, mtfA2, pRG3-AMA-NOT1-mtfA::pyr4; veA1 pyrG89; argB2, DnkuA::argB; pyroA4; veA+ argB2, DnkuA::argB; pyroA4; veA+ argB2, DnkuA::argB; veA+ pyrG89; argB2, DnkuA::argB; DmtfA::pyrGA.fum; pyroA4; veA+ DnkuA::argB; DmtfA::pyrGA.fum; veA+ pyrG89; argB2, DnkuA::argB, DmtfA::pyrGA.fum; pyroA::mtfA; pyroA4; veA+ pyrG89; argB2, DnkuA::argB, alcA(p)::mtfA::pyr4; pyroA4; veA+ pabaA1, pyrG89; DmftA::pyrGA.fum, DstcE::argB, DveA::argB pyrG89; DmftA::pyrGA.fum, DstcE::argB, DveA::argB DlaeA; veA+ veA1 DlaeA; veA1 pyrG89; wA3; argB2, DnkuA::argB; DmtfA::pyrGA.Phosphorylase kinase fum; DlaeA::methG; veA1 pyrG89; wA3; argB2, DnkuA::argB; DmtfA::pyrGA.fum; DlaeA::methG; veA+ pyrG89; DnkuA::argB; mtfA::gfp::pyrGA.fum; pyroASource FGSC [40] [40] [40] [40] [40] This study This study This study This study This study [71] This study This study This study This study This study This study This study This study [36] [39] [39] This study This study This studyFGSC, Fungal Genetics Stock Center.HBC doi:10.1371/journal.pone.0074122.tPLOS 1 | www.plosone.orgMtfA Controls Secondary Metabolism and DevelopmentMaterials and Techniques Fungal Strains and Growth ConditionsFungal strains utilized within this study are listed in Table 1. Media used contain glucose minimal media (GMM) [41], YGT (0.PMID:24140575 five yeast extract, 2 dextrose, trace elements ready as described [41], and oat meal media (OMM) (1 oat meal). Supplements for auxotrophic markers were added as required [41]. Glucose was substituted with threonine (100 mM) in threonine minimal medium (TMM) for induction of alcA promoter. Solid medium was prepared by adding ten g/liter agar. Strains had been stored as 30 glycerol stocks at 280uC.Genetic TechniquesMeiotic recombination in between A. nidulans strains was carried out as previously described [42]. Progeny in the cross between the RM7 mutant [40] and RAV2 (yA2, wA3, argB2, DstcE::argB, pyroA4) had been analyzed for the presence or absence of veA by PCR. Colony morphology, too as norsolorinic acid (NOR) production, had been also studied. The progeny of this cross showed four phenotypic groups: 1.
