anti-l-lactyllysine rabbit pab (bsa and azide free)

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Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free) Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Rat Synonyms: Kla Product Size 100 μg ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen L-lactylated lysine peptides MW (kDa) Multiple Specificity Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free) detects proteins post-translationally modified by lactylation on lysine residues. This pan antibody recognizes lactylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:1000 – 1:6000 Human, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. The antibody is now in 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. This BSA-, glycerol-, and azide-free antibody is particularly suitable for applications such as mass cytometry. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Peptide amount: 4 ng, 20 ng, 100ngExposure time: 10 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: (-): HeLa; (+): HeLa + sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μg Exposure time: 10 secondsPredicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human cerebrum Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:6000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free) Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Rat Synonyms: Kla Product Size 100 μg ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen L-lactylated lysine peptides MW (kDa) Multiple Specificity Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free) detects proteins post-translationally modified by lactylation on lysine residues. This pan antibody recognizes lactylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:1000 – 1:6000 Human, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. The antibody is now in 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. This BSA-, glycerol-, and azide-free antibody is particularly suitable for applications such as mass cytometry. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Peptide amount: 4 ng, 20 ng, 100ngExposure time: 10 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: (-): HeLa; (+): HeLa + sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μg Exposure time: 10 secondsPredicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human cerebrum Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:6000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with