anti-l-lactyllysine rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyllysine Rabbit mAb Clone Number: 9H1L6 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP ChIP Reactivity: All Synonyms: Kla Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen L-lactylated lysine peptides MW (kDa) Multiple Specificity Anti-L-Lactyllysine Rabbit mAb detects proteins with L-lactylated lysine residues. This pan antibody recognizes L-lactylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 – 1:100 Human FC 1:50 – 1:100 Human IP 1:25 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided below.Lane 1: L-lactylated peptide library. Lane 2: Acetylated peptide library. Lane 3: Propionylated peptide library. Lane 4: Butyrylated peptide library.Lane 5: Crotonylated peptide library. Lane 6: Malonylated peptide library. Lane 7: 2-Hydroxyisobutyrylated peptide library. Lane 8: Succinylated peptide library.Lane 9: Unmodified peptide library. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human colon cancerSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-L-Lactyllysine Rabbit mAb Clone Number: 9H1L6 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP ChIP Reactivity: All Synonyms: Kla Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen L-lactylated lysine peptides MW (kDa) Multiple Specificity Anti-L-Lactyllysine Rabbit mAb detects proteins with L-lactylated lysine residues. This pan antibody recognizes L-lactylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 – 1:100 Human FC 1:50 – 1:100 Human IP 1:25 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided below.Lane 1: L-lactylated peptide library. Lane 2: Acetylated peptide library. Lane 3: Propionylated peptide library. Lane 4: Butyrylated peptide library.Lane 5: Crotonylated peptide library. Lane 6: Malonylated peptide library. Lane 7: 2-Hydroxyisobutyrylated peptide library. Lane 8: Succinylated peptide library.Lane 9: Unmodified peptide library. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human colon cancerSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with