anti-l-lactyl-histone h4 (lys8) rabbit pab

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Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRLProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa ChIP Cell type: HeLaCross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 1:500, 1:200Beads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH Promoter, GAPDH CDS region, RPL30 Exon 3, FOXO3a-promoter and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.