anti-l-lactyl-histone h4 (lys8) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb Clone Number: 24H4L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP CUT&Tag Reactivity: Human, Mouse, Rat Synonyms: H4K8la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K8la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys8) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys8. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 4 μg/5×106 cells Human CUT&Tag 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, NIH/3T3, BRLProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa ChIP Cell type: HeLa+ sodium lactate (100 mM, 24 hours)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 4ug, 12ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, LDHA, RPL30 Exon 3, FOXO3a-promoter and FOXO3adownstream. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. CUT&Tag Cell type: HeLa + sodium lactate (100 mM, 24hr) Quantity of cell: 1×105 cells Primary ab dilution: 1: 100 Primary ab incubation condition: 4℃ overnight Secondary ab: Goat Anti-Rabbit IgG H&L pAb ( :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb Clone Number: 24H4L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP CUT&Tag Reactivity: Human, Mouse, Rat Synonyms: H4K8la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K8la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys8) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys8. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 4 μg/5×106 cells Human CUT&Tag 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, NIH/3T3, BRLProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa ChIP Cell type: HeLa+ sodium lactate (100 mM, 24 hours)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 4ug, 12ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, LDHA, RPL30 Exon 3, FOXO3a-promoter and FOXO3adownstream. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. CUT&Tag Cell type: HeLa + sodium lactate (100 mM, 24hr) Quantity of cell: 1×105 cells Primary ab dilution: 1: 100 Primary ab incubation condition: 4℃ overnight Secondary ab: Goat Anti-Rabbit IgG H&L pAb (