Product Name :
anti-l-lactyl-histone h4 (lys5) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H4K5la
UniProt ID :
P62805
Immunogen:
L-lactylated human histone H4 (Lys5) peptide
MW (kDa) :
11
Specificity:
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit pAb detects histone H4 only when it is L-lactylated at Lys5.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H4K5la, Lane 2: H4K5ac, Lane 3: H4K5pr, Lane 4: H4K5crLane 5: H4K5un, Lane 6: H4K5bhb, Lane 7: H4K8ac, Lane 8: H4K8prLane 9: H4K8cr, Lane 10: H4K8hib, Lane 11: H4K8bhb, Lane 12: H4K8laLane 13, H4K12ac, Lane 14: H4K12cr, Lane 15: H4K12hib, Lane 16: H4K12bhbLane 17: H4K12la, Lane 18: H4K5hib WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRLProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa
Vapor Pressure :
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（6） $ 420 Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb Catalog #
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（6） $ 420 Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb Catalog #
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