anti-l-lactyl-histone h4 (lys5) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb Clone Number: 17H1L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:100 Human FC 1:50 – 1:100 Human IP 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: H4K5 lactyl. Lane 2: H4K8 lactyl. Lane 3: H4K12 lactyl. Lane 4: H4K5 crotonyl. Lane 5: H4K8 crotonyl. Lane 6: H4K12 crotonyl. Lane 7: H4K8 2-hydroxyisobutyryl. Lane 8: H4K12 2-hydroxyisobutyryl. Lane 9: H4K5 unmodified. Lane 10: H4K5 2-hydroxyisobutyryl. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 11 kDa Observed band size: 11 kDa Lysates: HeLa, N2a, BRL Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ICC/IF Samples: (A) HeLa cells; (B) HeLa cells + sodium lactate (100 mM, 24 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb Clone Number: 17H1L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:100 Human FC 1:50 – 1:100 Human IP 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: H4K5 lactyl. Lane 2: H4K8 lactyl. Lane 3: H4K12 lactyl. Lane 4: H4K5 crotonyl. Lane 5: H4K8 crotonyl. Lane 6: H4K12 crotonyl. Lane 7: H4K8 2-hydroxyisobutyryl. Lane 8: H4K12 2-hydroxyisobutyryl. Lane 9: H4K5 unmodified. Lane 10: H4K5 2-hydroxyisobutyryl. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 11 kDa Observed band size: 11 kDa Lysates: HeLa, N2a, BRL Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ICC/IF Samples: (A) HeLa cells; (B) HeLa cells + sodium lactate (100 mM, 24 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:100 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Description: The green color represents the positive signal observed with