anti-l-lactyl-histone h4 (lys5) mouse mab

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Anti-L-Lactyl-Histone H4 (Lys5) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB FC Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys5) Mouse mAb detects histone H4 only when it is L-lactylated at Lys5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat FC 1:50 – 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: N2a, BRL, Mouse spleenProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:100Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (Black line).Isotype control: Mouse monoclonal IgG (Blue line).Description: Line red is the positive signal for :
Anti-L-Lactyl-Histone H4 (Lys5) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB FC Reactivity: Human, Mouse, Rat Synonyms: H4K5la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys5) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys5) Mouse mAb detects histone H4 only when it is L-lactylated at Lys5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat FC 1:50 – 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: N2a, BRL, Mouse spleenProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 11 kDaObserved band size: 11 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:100Secondary Ab: Goat Anti-Mouse IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (Black line).Isotype control: Mouse monoclonal IgG (Blue line).Description: Line red is the positive signal for