anti-l-lactyl-histone h4 (lys16) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb Clone Number: 2H2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K16la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys16) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys16. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human ICC/IF 1:500 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation is a recently discovered post-translational modification that is induced by lactate, a metabolite produced during glycolysis. Regulated by glucose metabolic dynamics and lactate levels within the cellular microenvironment, histone lactylation is implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Notably, Sirtuin 3 (SIRT3) has been reported as an eraser of H4K16 lactylation (H4K16la). Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds The list of peptides used in the experiment is provided in the table below. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb Clone Number: 2H2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K16la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16la UniProt ID P62805 Immunogen L-lactylated human histone H4 (Lys16) peptide MW (kDa) 11 Specificity Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys16. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human ICC/IF 1:500 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation is a recently discovered post-translational modification that is induced by lactate, a metabolite produced during glycolysis. Regulated by glucose metabolic dynamics and lactate levels within the cellular microenvironment, histone lactylation is implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Notably, Sirtuin 3 (SIRT3) has been reported as an eraser of H4K16 lactylation (H4K16la). Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds The list of peptides used in the experiment is provided in the table below. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with