anti-l-lactyl-histone h3 (lys56) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys56) Rabbit mAb Clone Number: 38H5L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB FC Reactivity: Human Synonyms: H3K56la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K56la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys56) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1: 1000 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Secondary Ab: Goat Anti-Rabbit IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys56) Rabbit mAb Clone Number: 38H5L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB FC Reactivity: Human Synonyms: H3K56la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K56la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys56) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1: 1000 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Secondary Ab: Goat Anti-Rabbit IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for