anti-l-lactyl-histone h3 (lys23) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb Clone Number: PA-148(B)-213 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb detects endogenous levels of histone H3 when it is lactylated at Lys23. This antibody shows minor cross-reactivity to L-lactyl-histone H3 (Lys18) peptide. Product Usage Information Applications Dilution Recommended Species WB 1:2000 – 1:10000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human ICC/IF 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23 lactyl. Lane 2: H3K18 lactyl. Dot 3: H3K23 acetyl. Dot 4: H3K23 crotonyl. Dot 5: H3K23 butyryl. Dot 6: H3K23 protonyl. Dot 7: H3K23 unmodified. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. Lysates: (-): NIH-3T3 cells; (+): NIH-3T3 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. Lysates: (-): C6 cells; (+): C6 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. IHC-P Tissue: Human colonSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb Clone Number: PA-148(B)-213 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb detects endogenous levels of histone H3 when it is lactylated at Lys23. This antibody shows minor cross-reactivity to L-lactyl-histone H3 (Lys18) peptide. Product Usage Information Applications Dilution Recommended Species WB 1:2000 – 1:10000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human ICC/IF 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23 lactyl. Lane 2: H3K18 lactyl. Dot 3: H3K23 acetyl. Dot 4: H3K23 crotonyl. Dot 5: H3K23 butyryl. Dot 6: H3K23 protonyl. Dot 7: H3K23 unmodified. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. Lysates: (-): NIH-3T3 cells; (+): NIH-3T3 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. Lysates: (-): C6 cells; (+): C6 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 2.5% BSA/TBST Primary Ab dilution: 1:10000 (2.5% BSA) Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDaImportant Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands. IHC-P Tissue: Human colonSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with