Product Name :
anti-l-lactyl-histone h3 (lys18) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K18la
UniProt ID :
P68431
Immunogen:
Lactylated human histone H3 (Lys18) peptide
MW (kDa) :
15
Specificity:
Anti-Lactyl-Histone H3 (Lys18) Rabbit pAb detects histone H3 only when it is lactylated at Lys18. This antibody has been shown to selectively recognize lactylated H3 peptide at Lys18, but not the acetylated, crotonylated, butyrylated, propionylated, or unmodified H3 peptide at Lys18.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subject to a variety of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. The Warburg effect, initially characterized by an increase in lactate production in cancer cells, is linked to various cellular processes such as angiogenesis, hypoxia, polarization of macrophages, and T cell activation. This phenomenon is closely associated with several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is well-known as an energy source and metabolic byproduct. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification derived from the cellular metabolite lactate. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below.https://s2.loli.net/2022/08/08/BQ34Cpz8EmTZisK.png WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa; (+) HeLa + sodium lactate (100 mM, 24 h)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: N2a, BRLProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:100Secondary Ab: Goat anti-Rabbit IgGUnlabeled control: Cells without incubation with primary and secondary antibodies (black line).Isotype control: Rabbit monoclonal IgG (blue line).Comment: Line red is the positive signal for
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18la Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 420 Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb Catalog #
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18la Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 420 Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb Catalog #
Related websites: https://www.medchemexpress.com/antibodies.html
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