Product Name :
anti-l-lactyl-histone h3 (lys18) rabbit mab (chip grade)
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K18la
UniProt ID :
P68431
Immunogen:
L-lactylated human histone H3 (Lys18) peptide
MW (kDa) :
15
Specificity:
Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade) detects histone H3 only when it is L-lactylated at Lys18.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K18 lactyl. Lane 2: H3K18 acetyl. Lane 3: H3K18 crotonyl.Lane 4: H3K18 butyryl. Lane 5: H3K18 propionyl. Lane 6: H3K18 2-hydroxyisobutyryl.Lane 7: H3K18 β-hydroxybutyryl. Lane 8: H3K18 unmodified WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDaObserved band size: 15 kDa Lysates: (-): K562 cells; (+): K562 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDaObserved band size: 15 kDa Lysates: (-): NIH/3T3 cells; (+): NIH/3T3 cells + sodium lactate (25 mM, 24 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDaObserved band size: 15 kDa ChIP Sample: Hela cells + sodium lactate (100 mM, 24 hours)Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS, LDHA-promoter, LDHA, FOXO3a-downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon. CUT&Tag Sample: HeLa cells + sodium lactate (100 mM, 24 hours) Cell quantity: 1×105 cells Primary Ab dilution: 1:100 Primary Ab incubation condition: 4⁰C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade) Clone Number: PA
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade) Clone Number: PA
Related websites: https://www.medchemexpress.com/antibodies.html
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