T HCT-116 (human colon cancer) cell lines. The compound 14 was found to become inhibitive against Panc-1 (human pancreatic adenocarcinoma) as well as HCT-116 (human colon cancer) cell lines, and hence, further investigations are in have to have in these promising lead molecules. Added fileAdditional file 1: Spectral evidences. A copy of original 1H NMR and 13 C NMR spectra of the compounds 1 to 26 has been integrated. Competing interests The authors declare that they have no competing interests. Acknowledgements The authors are grateful to Syngene International Pvt. Ltd., Bengaluru for delivering spectral facilities. They are also thankful towards the VIT management for their generous help and facilities. Author specifics 1 Centre for Organic and Medicinal Chemistry, VIT University, Vellore 632 014, India. 2Medical and Biological Computing Laboratory, College of Biosciences and Technologies, VIT University, Vellore 632 014, India. 3IndustrialPurified by recrystallisation utilizing ethanol, m.p: 215.five to 216.8 (white solid).1H NMR (400 MHz, d6-DMSO) H: 0.58 to 0.55 (m, 2H, protons of cyclopropyl), 0.85 to 0.81 (m, 2H, protons of cyclopropyl), 1.75 to 1.68 (m, 1H, proton of cyclopropyl), 9.50 (bs, 1H, -NH proton), 11.52 (bs, 1H, -OH proton); 13C NMR (100 MHz, d6-DMSO): 7.27 (C-1 of cyclopropyl ring), 7.59 (C-2, three of cyclopropylRagavan et al. Organic and Medicinal Chemistry Letters 2013, 3:6 http://www.orgmedchemlett/content/3/1/Page 14 ofBiotechnology Division, School of Bio Sciences and Technologies, VIT University, Vellore 632 014, India. 4Department of Oncology, HCS HTS, Piramal Life Sciences Ltd. Guregaon (E), Mumbai 400063, India. 5Department of Biochemistry, K.S. Hegde Health-related Academy, Deralakatte 574 162, India. Received: 30 April 2013 Accepted: 19 June 2013 Published: 19 JulyReferences 1. Ueda TH, Mase N, Oda II (1981) Synthesis of pyrazolone derivatives. XXXIX. Synthesis and analgesic activity of pyrano[2,3,-c]pyrazoles. Chem Pharm Bull 29:3522528 2. Hukki J, Laitinen P, Alberty JE (1968) Preparation and pharmacological activity of pyrazole derivatives with possible antihistaminic properties II. An attempted synthesis of 1-phenyl and 1-benzyl-3-methyl-5-pyrazolones aminoalkylated at position two. Pharm Acta Helv 43:70412 three. Nakagawa H, Ohyama R, Kimata A, Suzuki T, Miyata N (2006) Hydroxyl radical scavenging by edaravone derivatives: effective scavenging by 3-methyl-1-(pyridine-2-yl)-5-pyrazolone with an intramolecular base. Bioorg Med Chem Lett 16:5939942 4. Kawai H, Nakai H, Suga M, Yuki S, Watanabe T, Saito KI (1997) Effects of a novel absolutely free radical scavenger, MCI-186, on ischemic brain harm in the rat distal middle cerebral artery occlusion model.Hesperidin J Pharmacol Exp Ther 281:92127 5.Ublituximab Watanabe T, Yuki S, Egawa M, Nishi H (1984) Protective effects of MCI-186 on cerebral ischemia: possible involvement of free of charge radical scavenging and antioxidant actions.PMID:23672196 J Pharmacol Exp Ther 268:1597604 6. Wu TW, Zeng LH, Wu J, Fung KP (2002) Myocardial protection of MCI-186 in rabbit ischemia-reperfusion. Life Sci 71:2249255 7. Barba O, Jones LH (2004) Pyrazole derivatives as reverse transcriptase inhibitors.. WO Patent 029042 A1, 8 Apr 2004 eight. Plath P, Rohr W, Wuerzer B (1980) Herbicides containing 3-aryl-5methylpyrazole-4-carboxylic acid esters. Ger Offen. DE 2920933 A1 1801204, 4 Dec 1980 9. Cativiela C, Serrano JL, Zurbano MM (1995) Synthesis of 3-substituted pentene-2,4-diones: beneficial intermediates for liquid crystals. J Org Chem 60:3074083 10. Sugiura S, Ohno.
