anti-l-lactyl-histone h2b (lys16) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H2B (Lys16) Rabbit mAb Clone Number: PA-024-15 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK16la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK16la UniProt ID P62807 Immunogen L-lactylated human Histone H2B (Lys16) peptide MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1ng, 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa+ sodium lactate (100 mM, 24 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL, 4: Recombinant histone H2B (20 ng)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H2B (Lys16) Rabbit mAb Clone Number: PA-024-15 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK16la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK16la UniProt ID P62807 Immunogen L-lactylated human Histone H2B (Lys16) peptide MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1ng, 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa+ sodium lactate (100 mM, 24 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL, 4: Recombinant histone H2B (20 ng)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa