anti-l-lactyl-histone h2b (lys15) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H2B (Lys15) Rabbit mAb Clone Number: PD-025-55 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human, Mouse Synonyms: H2BK15la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK15la UniProt ID P62807 Immunogen L-lactylated human histone H2B (Lys15) peptide MW (kDa) 14 Specificity Anti-L-Lactyl-Histone H2B (Lys15) Rabbit mAb detects histone H2B only when it is L-lactylated at Lys15. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 1ng, 4 ng, 16 ng, 64 ng; 2-7: 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa; (+): HeLa + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): K562; (+): K562 + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): NIH/3T3; (+): NIH/3T3 + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H2B (Lys15) Rabbit mAb Clone Number: PD-025-55 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: Human, Mouse Synonyms: H2BK15la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK15la UniProt ID P62807 Immunogen L-lactylated human histone H2B (Lys15) peptide MW (kDa) 14 Specificity Anti-L-Lactyl-Histone H2B (Lys15) Rabbit mAb detects histone H2B only when it is L-lactylated at Lys15. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 1ng, 4 ng, 16 ng, 64 ng; 2-7: 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa; (+): HeLa + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): K562; (+): K562 + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): NIH/3T3; (+): NIH/3T3 + sodium L-lactate (25 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa