anti-keratin 20 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Keratin 20 Rabbit mAb Clone Number: JRMR-10022(2)-72 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CK-20, KRT20, Cytokeratin-20 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CK-20, KRT20, Cytokeratin-20 UniProt ID P35900 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Rat, Mouse IHC-P 1:100 – 1:500 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Keratin (cytokeratin) is an intermediate filament protein, which is mainly expressed in epithelial cells such as gastric epithelium and intestinal epithelium. Keratin heterodimers composed of acidic keratin (or type I keratin, keratin 9 to 23) and basic keratin (or type II keratin, keratin 1 to 8) assemble to form filaments. Keratin isoforms exhibit tissue- and differentiation-specific characteristics, making them useful as research biomarkers. Studies have shown that mutations in the keratin gene are related to skin diseases, liver and pancreatic diseases, and inflammatory bowel disease. Therefore, cytokeratin can be used as a tumor immunohistochemical marker.Keratin 20 (CK-20) is an essential intermediate filament component and the main cytoskeleton keratin of the intestinal epithelium. Studies have shown that keratin 20 is an important marker for colon cancer, liver cancer, and stomach cancer. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1. SW480; 2. HCT-116; 3. HT_x0002_29; 4. Mouse small intestine; 5. Mouse colon; 6. Rat colonProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 48 kDaObserved band size: 48 kDa IHC-P Tissue: Human colonSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Keratin 20 Rabbit mAb Clone Number: JRMR-10022(2)-72 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CK-20, KRT20, Cytokeratin-20 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CK-20, KRT20, Cytokeratin-20 UniProt ID P35900 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Rat, Mouse IHC-P 1:100 – 1:500 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Keratin (cytokeratin) is an intermediate filament protein, which is mainly expressed in epithelial cells such as gastric epithelium and intestinal epithelium. Keratin heterodimers composed of acidic keratin (or type I keratin, keratin 9 to 23) and basic keratin (or type II keratin, keratin 1 to 8) assemble to form filaments. Keratin isoforms exhibit tissue- and differentiation-specific characteristics, making them useful as research biomarkers. Studies have shown that mutations in the keratin gene are related to skin diseases, liver and pancreatic diseases, and inflammatory bowel disease. Therefore, cytokeratin can be used as a tumor immunohistochemical marker.Keratin 20 (CK-20) is an essential intermediate filament component and the main cytoskeleton keratin of the intestinal epithelium. Studies have shown that keratin 20 is an important marker for colon cancer, liver cancer, and stomach cancer. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1. SW480; 2. HCT-116; 3. HT_x0002_29; 4. Mouse small intestine; 5. Mouse colon; 6. Rat colonProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 48 kDaObserved band size: 48 kDa IHC-P Tissue: Human colonSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with