anti-keratin 14 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Keratin 14 Rabbit mAb Clone Number: JRMR-10985-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: Cytokeratin-14, CK-14, K14, Krt14 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Cytokeratin-14, CK-14, K14, Krt14 UniProt ID Q61781 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:100 – 1:500 Human FC 1:500 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Keratin 14 is a type I cytokeratin, a group of acidic intermediate filament proteins. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Mouse heart (Negative control), 2: Mouse skin, 3: Rat skin Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 53 kDa Observed band size: 53 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: SY-SH5Y (Negative control), 2: A431Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 53 kDa Observed band size: 53 kDa IHC-P Tissue: Human prostatic hyperplasia Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Keratin 14 Rabbit mAb Clone Number: JRMR-10985-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: Cytokeratin-14, CK-14, K14, Krt14 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Cytokeratin-14, CK-14, K14, Krt14 UniProt ID Q61781 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:100 – 1:500 Human FC 1:500 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Keratin 14 is a type I cytokeratin, a group of acidic intermediate filament proteins. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Mouse heart (Negative control), 2: Mouse skin, 3: Rat skin Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 53 kDa Observed band size: 53 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: SY-SH5Y (Negative control), 2: A431Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 53 kDa Observed band size: 53 kDa IHC-P Tissue: Human prostatic hyperplasia Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with