anti-isg20 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-ISG20 Rabbit mAb Clone Number: JRMR-3130 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: HEM45 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HEM45 UniProt ID Q96AZ6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interferon-stimulated 20 kDa protein (ISG20) is an exoribonuclease that functions as part of the host innate immune response to RNA-based viruses. Indeed, expression of ISG20 can be induced by type-I and type-II interferons following cellular exposure to RNA-based viruses. Mechanistically, ISG20 localizes to the nucleolus and is believed to inhibit the replication of RNA-based viruses, in part, by employing 3′-5′ exonuclease activity toward viral RNAs. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Raji; 2: Daudi Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 20 kDa Observed band size: 17 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-ISG20 Rabbit mAb Clone Number: JRMR-3130 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: HEM45 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HEM45 UniProt ID Q96AZ6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interferon-stimulated 20 kDa protein (ISG20) is an exoribonuclease that functions as part of the host innate immune response to RNA-based viruses. Indeed, expression of ISG20 can be induced by type-I and type-II interferons following cellular exposure to RNA-based viruses. Mechanistically, ISG20 localizes to the nucleolus and is believed to inhibit the replication of RNA-based viruses, in part, by employing 3′-5′ exonuclease activity toward viral RNAs. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Raji; 2: Daudi Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 20 kDa Observed band size: 17 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with