anti-hsp70 rabbit pab

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Anti-HSP70 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat IHC-P 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 70 (HSP70) is a ubiquitous and highly conserved protein. As a chaperone molecule, HSP70 can promote the correct folding of proteins; HSP70 participates in cell apoptosis, by preventing the release of cytochrome C from mitochondria, binding with apoptosisinducing factors to prevent it from entering the nucleus, or inhibiting the activity of JNK kinase to regulate cell apoptosis; HSP70 also It can regulate the cell cycle process, promote cell growth and prevent cell senescence. In addition, HSP70 can be used as an immune adjuvant to participate in synergistic immunity and stimulate antigen-specific CTL responses. Cellular location Cytoskeleton, Cytoplasm, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: A549, 3: 293T, 4: MCF-7Protein loading quantity: 20 μgExposure time: 1 sPredicted band size: 70 kDaObserved band size: 70 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse kidney, 2: Rat kidneyProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 70 kDaObserved band size: 70 kDa IHC-P Tissue: Human breast carcinomaSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:50Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-HSP70 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat IHC-P 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 70 (HSP70) is a ubiquitous and highly conserved protein. As a chaperone molecule, HSP70 can promote the correct folding of proteins; HSP70 participates in cell apoptosis, by preventing the release of cytochrome C from mitochondria, binding with apoptosisinducing factors to prevent it from entering the nucleus, or inhibiting the activity of JNK kinase to regulate cell apoptosis; HSP70 also It can regulate the cell cycle process, promote cell growth and prevent cell senescence. In addition, HSP70 can be used as an immune adjuvant to participate in synergistic immunity and stimulate antigen-specific CTL responses. Cellular location Cytoskeleton, Cytoplasm, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: A549, 3: 293T, 4: MCF-7Protein loading quantity: 20 μgExposure time: 1 sPredicted band size: 70 kDaObserved band size: 70 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse kidney, 2: Rat kidneyProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 70 kDaObserved band size: 70 kDa IHC-P Tissue: Human breast carcinomaSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:50Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with