anti-hsd17b1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-HSD17B1 Rabbit mAb Clone Number: JRMR-10632-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20-alpha-HSD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20-alpha-HSD UniProt ID P14061 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 Human IHC-P 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HSD17B1 is a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. HSD17B1 catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. HSD17B1 is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:20000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Human heart (Negative control), 2: Human placenta Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 35 kDa Observed band size: 35 kDa IHC-P Tissue: Human placenta Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-HSD17B1 Rabbit mAb Clone Number: JRMR-10632-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20-alpha-HSD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20-alpha-HSD UniProt ID P14061 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 Human IHC-P 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HSD17B1 is a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. HSD17B1 catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. HSD17B1 is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:20000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Human heart (Negative control), 2: Human placenta Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 35 kDa Observed band size: 35 kDa IHC-P Tissue: Human placenta Section type: Formalin-fixed &Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with