anti-hp1 gamma mouse mab

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Recombinant Anti-HP1 gamma Mouse mAb Clone Number: JMMR-1208 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: HECH, CBX3, Modifier 2 protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HECH, CBX3, Modifier 2 protein UniProt ID Q13185 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:1000 – 1:5000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HP1 gamma (HP1γ) is a member of Heterochromatin protein 1 (HP1) family, which is involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 Trimethylated at Lys9, a histone “mark” closely associated with centromeric heterochromatin. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HEK-293T, 2: HeLa, 3: F9 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 21 kDa Observed band size: 21 kDa IHC-P Tissue: Human testis Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature andhighpressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-HP1 gamma Mouse mAb Clone Number: JMMR-1208 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: HECH, CBX3, Modifier 2 protein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HECH, CBX3, Modifier 2 protein UniProt ID Q13185 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:1000 – 1:5000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HP1 gamma (HP1γ) is a member of Heterochromatin protein 1 (HP1) family, which is involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 Trimethylated at Lys9, a histone “mark” closely associated with centromeric heterochromatin. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HEK-293T, 2: HeLa, 3: F9 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 21 kDa Observed band size: 21 kDa IHC-P Tissue: Human testis Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature andhighpressure Retrieval buffer: Tris/EDTA buffer, pH9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with