anti-hmgcr rabbit mab

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Recombinant Rabbit Monoclonal Anti-HMGCR Rabbit mAb Clone Number: JRMR-10374-41 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse Synonyms: HMG-CoA Reductase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HMG-CoA Reductase UniProt ID P04035 Immunogen Recombinant human HMG-CoA Reductase fragment MW (kDa) 97 Specificity Anti-HMG-CoA Reductase Rabbit mAb recognizes endogeneous level of HMG-CoA Reductase protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Mouse FC 1:50 – 1:100 Human IP 1:25 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is a transmembrane glycoprotein of the endoplasmic reticulum. It is the rate-limiting enzyme for cholesterol biosynthesis and non-sterol isoprenoid biosynthesis. Play a key role in cellular cholesterol homeostasis. The activity of HMGCR is regulated by negative feedback, in which cholesterol and other end products of the metabolic pathway inhibit the activity of HMGCR in a multivalent manner. Cholesterol inhibits reductase activity mainly by inhibiting the transcription rate of HMGCR gene. HMGCR is the main target of statins, which are a class of cholesterol-lowering drugs. Cellular location Endoplasmic reticulum, Peroxisome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: HepG2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 97 kDaObserved band size: 97 kDa ICC/IF Cell line: NIH-3T3Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°CovernightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-HMGCR Rabbit mAb Clone Number: JRMR-10374-41 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse Synonyms: HMG-CoA Reductase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HMG-CoA Reductase UniProt ID P04035 Immunogen Recombinant human HMG-CoA Reductase fragment MW (kDa) 97 Specificity Anti-HMG-CoA Reductase Rabbit mAb recognizes endogeneous level of HMG-CoA Reductase protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Mouse FC 1:50 – 1:100 Human IP 1:25 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is a transmembrane glycoprotein of the endoplasmic reticulum. It is the rate-limiting enzyme for cholesterol biosynthesis and non-sterol isoprenoid biosynthesis. Play a key role in cellular cholesterol homeostasis. The activity of HMGCR is regulated by negative feedback, in which cholesterol and other end products of the metabolic pathway inhibit the activity of HMGCR in a multivalent manner. Cholesterol inhibits reductase activity mainly by inhibiting the transcription rate of HMGCR gene. HMGCR is the main target of statins, which are a class of cholesterol-lowering drugs. Cellular location Endoplasmic reticulum, Peroxisome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: HepG2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 97 kDaObserved band size: 97 kDa ICC/IF Cell line: NIH-3T3Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°CovernightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with