Fungal ITS of eight genera of Ascomycota, five genera of Basidiomycota, Rhizopodium (Chytridiomycota), and Mortierella (Fungi incertae sedis) (Table 2). Bands 9 and 15, of which the DNA was most closely connected to the genera Davidiella and Rhizophydium, respectively, have been related with J2 from all three soils, although they wereFIG 1 DGGE profiles of fungal ITS fragments amplified from DNA of M. hapla J2 from three arable soils and from total soil DNA. Fungal ITS sorts are marked that were NOP Receptor/ORL1 web enriched in nematode samples and characterized by sequencing (Table two). A, B, C, and D refer to replicate soil baiting assays for each and every soil.Might 2014 Volume 80 Numberaem.asm.orgAdam et al.TABLE 2 Identification and frequency with the dominant nematode-specific DGGE bandsNo. of samples where band was discovered Nematodes DGGE sort and band no. Fungus DGGE 1 two 3 4 5 six 7 8 9 ten 11 12 13 14 15 Bacillus DGGE 1 two 3 4 5 6 7 8 9 ten Alphaproteobacterium DGGE 1 2 3 4 five six 7 8 9 10 11 12 13 Pseudomonas DGGE 1 two three four five 6aSoil Gb 4 two 0 0 0 0 0 four four four 0 2 0 4 two Kw four 0 0 0 0 0 2 0 0 0 0 four 0 four 0 Go 4 0 0 0 four 0 0 0 0 four 0 four 0 four 0 Gb four 0 0 0 0 0 0 four 0 4 0 4 0 4Closest GenBank match (organism, GenBank no.) Malassezia restricta, EU400587 Aspergillus penicillioides, GU017496 Cryptococcus pseudolongus, AB105353 Chaetomium globosum, JX501299 Arthopyreniaceae, FJ439584 Eurotium sp., AM901702 Ganoderma applanatum, JX501311 Cladosporinum cladosporioides, AJ300335 Davidiella sp., JX164064 Cryptococcus sp., JX164076 Trichosporonales, EF060720 Mortierella sp., JF439489 Cylindrocarpon olidum, GU198183 Ascomycete, AM410609 Rhizophydium sp., DQaidentity 98.7 99.6 100 98.2 100 one hundred 99.six one hundred 99.six 100.0 98.3 99.6 99.0 99.two 98.Kw four four 0 0 0 4 four 0 four 0 four four four 4Go four 0 4 4 four 0 0 0 four 0 4 four 0 4Bradyrhizobium pachyrhizi, NR_043037 Sphingomonas insulae, NR_044187 Staphylococcus epidermidis, NR_036904 Staphylococcus epidermidis, NR_036904 Micrococcus endophyticus, NR_044365 Bacillus megaterium, NR_043401 Micrococcus luteus, NR_037113 Propionibacterium acnes, NR_040847 Methylobacterium rhodesianum, NR_041028 Streptococcus thermophilus, NR_97.9 99.4 one hundred 99.6 98.six 99.7 99.2 one hundred 97.20 1 four four three four 4 four 23 1 four 4 3 4 four four 10 3 4 four four 4 4 four 30 0 0 0 0 0 four 4 00 0 0 0 0 0 4 4 00 0 0 0 0 0 four four 0Solirubrobacter soli, NR_041365 Janthinobacterium lividum, NR_026365 Rhizobium phaseoli, NR_044112 Pedomicrobium australicum, NR_026337 Ochrobactrum anthropi, NR_074243 Hyphomonadaceae, NR_041967 Anaplastic lymphoma kinase (ALK) Inhibitor MedChemExpress Nitrospira moscoviensis, NR_029287 Rhodobiaceae, NR_042626 Devosia chinhatensis, NR_044214 Kaistia soli, NR_044302 Magnetospirillum gryphiswaldense, NR_027605 Bosea eneae, NR_028798 Rhodobacter blasticus, NR_99.eight 99.eight 99.eight 96.0 99.five 91.0 96.three 92.eight 96.6 96.0 96.three 95.five 96.two 1 four 1 four three 2 two 0 0 1 43 0 4 3 three two 3 two three two 3 41 three four three two 3 0 2 two 3 1 43 0 0 4 2 4 0 0 four 0 0 03 0 0 4 four four 0 0 four 0 0 00 0 0 4 0 four 0 0 4 0 0 0Pseudomonas asplenii, NR_040802 Pseudomonas tuomuerensis, NR_043990 Pseudomonas koreensis, NR_025228 Pseudomonas jessenii, NR_024918 Pseudomonas jessenii, NR_024918 Pseudomonas koreensis, NR_025228 Pseudomonas taetrolens, NR_99.5 99.1 100 99.3 99.1 99.8 98.0 two three 1 1 33 three 0 3 1 00 2 0 three three ten 4 0 0 0 00 4 0 4 0 00 4 0 three four 0Details for the BLASTN final results and taxonomy are given in the supplemental material. (T), type strain.mostly below the detection limit inside the soil fungal communities. Some bands have been popular on both nematodes and soil samples inside the 3 soils, which include bands 1, 12, and 14, which corresponded to Malassezia restricta, Morti.
